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Table 1 PCR primers and thermal cycling conditions

From: Methylation of the KEAP1 gene promoter region in human colorectal cancer

Methods Primers Sequence
BSP   Forward: 5'-AAGAAAAGAAAAGAAAAGAAAATTTAG-3'
   Reverse: 5'-TTTAGTGAGGTAGATAATTTTTT-3'
PCR conditions   Initial denaturation at 95°C (10 min) and 35 cycles at 95°C (3 s), 52°C (3 s), 72°C (5 s), and a final extension at 72°C (10 s)
MSP Methylation- Forward: 5'-TAGATAATTTTTTTTAGATTTTGCGGTCG-3'
  Specific Reverse: 5'-TCCTCGCGAAACTACGC-3'
PCR condition   Initial denaturation at 95°C (10 min) and annealing temperature decrement of 0.5°C every cycle (from 70°C to 66.5°C) followed by 32 cycles of 66°C (3 s), 72°C (5 s), and a final extension at 72°C (10 s)
MSP Non-methylation Forward: 5'-TAGATAATTTTTTTTAGATTTTGTGGTTG-3'
  -specific Reverse: 5'-TCCTCACAAAACTACAC-3'
PCR condition   Initial denaturation at 95°C (10 min) and annealing temperature decrement of 0.5°C every cycle (from 64°C to 60.5°C) followed by 32 cycles of 60°C(3 s), 72°C (5 s), and a final extension at 72°C (10 s)