Endogenous expression and localization of BCA2 and partner proteins. [A] Western blot comparing expressed protein levels of BCA2, hHR23a, 14-3-3σ and β-Tubulin in ER-positive and ER-Negative cell lines. Normalized relative intensities of each protein are displayed below their respective blots. Microscopy images, taken at 100 × magnification, of endogenous IF staining for [B] BCA2 and hHR23a or [C] BCA2 and 14-3-3σ, tagged with either secondary antibody conjugated to FITC-dye or Cy3, in MCF7 breast cancer cells. The last panel shows the merged image and co-localization of the proteins.