Figure 3

GPER/GPR30 expression in human lung and breast tumors. The TMA slides, ‘Multiple lung cancer test array with unmatched normal adjacent tissue’ (US BioMax Inc, #LC242) and ‘human breast cancer test tissue array with self-matching or unmatched normal adjacent tissues’ (US Biomax Inc #BR241) were stained with H&E and anti-GPR30 (10 μg/ml). (A-B). The representative tissue spot/core images of Hand E (A) and GPR30 (B) staining for lung cancer (adenocarcinoma) are shown. (C-D). The representative normal lung tissue for H and E (C) and GPR30 staining (D) are shown. (E-F) The breast cancer TMA slides were stained with H&E and anti-GPR30 as a positive control for GPR30 expression. The images were collected at 200X magnification using Aperio image scope. The scale bars indicate 100 μm.