Figure 4
From: The role of brevican in glioma: promoting tumor cell motility in vitro and in vivo
The motility of glioma cells was decreased by brevican knockdown. (A) A wound healing assay showed that the spreading ability in the transduced U251/brevican-shDNA 2 cells was significantly inhibited by brevican knockdown at 24 and 48 h. (B) The live transduced cells were incubated with anti-brevican antibody and subsequently processed for immunoassay. Cytoimmunofluorescence analyses also indicated the cell infiltrating ability was inhibited and brevican expression was decreased in the transduced U251/brevican-shDNA 2 cells. Brevican expression was reduced in the cytoplasm of glioma cells. TU, the transduced U251 cells; TU-shDNA mock, the transduced U251/brevican-mock cells; TU-shDNA2, the transduced U251/ brevican-shDNA 2 cells. * P < 0.05; ** P < 0.01.