Figure 3

Brevican knockdown reduced glioma cell proliferation and invasion. (A) Brevican knockdown effectively down-regulated brevican expression. Western blot analysis of cell lysates demonstrated down-regulation of brevican protein in the transduced U251/brevican-shDNA 2 and the transduced U251/brevican-shDNA 3 cells by 52.3% and 23.4%, respectively. (B) Optical microscopic observation showed that cell colony formation was inhibited in the transduced U251/brevican-shDNA 2 cells (c), compared with the transduced U251 cells (a) and the control cells (b). (C) The rate of cell invasion was also reduced in the transduced U251/brevican-shDNA 2 cells (c) compared with the transduced U251 cells (a) and control cells (b). TU, the transduced U251 cells (a); TU-shDNA mock, the transduced U251/brevican-shDNA mock cells (b); TU-shDNA2, the transduced U251/brevican- shDNA 2 cells (c). ** P < 0.01.