Brevican expression levels were detected by immunohistochemistry staining using an anti-brevican antibody. (A) The staining results were observed by microscopy. Meningioma was negative (a, blue; n = 20), pituitary adenoma was weakly-positive (b, light yellow; n = 20) and malignant glioma sections (astrocytoma grades I-IV, n = 15 for each) were positive (c- f, yellow to brown; respectively). Hematoxylin was used for nuclear counterstaining (blue). Left: 100×, HE staining (above), IHC (lower); right: 400×, magnification of square frame sections in the left; (B) The immunohistochemistry PI of brevican expression in the gliomas (5.27 ± 1.98), especially for grade III and IV astrocytoma (6.07 ± 2.30 and 8.07± 2.22), was significantly higher than that of benign brain tumors (1.78 ± 0.89). *,P < 0.05; ** P < 0.01.