Table 2 Information of the oligonucleotides used for the qRT-PCR analysis
Gene Name | Sequence Accession Number | Primer Sequence | Primer Location (Exon | Prod. Length | T°a | |
|---|---|---|---|---|---|---|
WNT7A | Wingless-type MMTV integration site family, member 7A | NM_004625 | F CAAAGAGAAGCAAGGCCAGTA R GTAGCCCAGCTCCCGAAACTG | 707-730 (3) 962-983 (4) | 277 | 60 |
MYC | V-myc myelocytomatosis viral oncogene homolog (avian) | NM_002467.4 | F CCAGCGCCTTCTCTCCGTC R GGGAGGCGCTGCGTAGTTGT | 1208-1226 (2) 1490-1509 (3) | 302 | 60 |
JUN | Jun proto-oncogene | NM_002228.3 | F TGGAAAGTACTCCCCTAACCT R CTGAAACATCGCACTATCCTT | 2786-2806 (1) 3015-3035 (1) | 250 | 60 |
FRA-1 | FOS-like antigen 1 | NM_005438.3 | F AGGAACCGGAGGAAGGAACTG R TGCCACTGGTACTGCCTGTGT | 554-574 (3) 732-752 (4) | 199 | 60 |
AXIN2 | Axin 2 | NM_004655.3 | F AAAAAGGGAAATTATAGGTATTAC R CGATTCTTCCTTAGACTTTG | 2678-2701 (10/11) 2935-2954 (11) | 277 | 54 |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | NM_002046.3 | F CACTGCCACCCAGAAGACTGTG R TGTAGGCCATGAGGTCCACCAC | 645-666 (8) 1072-1089 (9) | 449 | 63 |
RPL32 | Ribosomal protein L32 | NM_000994.3 | F GACTTGACAACAGGGTTCGTAG R ATTTAAACAGAAAACGTGCACA | 213-234 (3) 511-532 (4) | 320 | 60 |
RPS18 | Ribosomal protein S18 | NM_022551.2 | F CGATGGGCGGCGGAAAA R CAGTCGCTCCAGGTCTTCACGG | 105-121 (2) 366-387 (5) | 283 | 58 |
B2M | Beta-2-microglobulin | NM_004048.2 | F GAGGCTATCCAGCGTACTCCAA R CACACGGCAGGCATACTCAT | 115-136 (1/2) 347-366 (2) | 252 | 58 |
ACTB | Actin beta | NM_001101.3 | F TCCGCAAAGACCTGTACG R AAGAAAGGGTGTAACGCAACTA | 950-967 (5) 1226-1247 (6) | 298 | 60 |