Table 2 Information of the oligonucleotides used for the qRT-PCR analysis
| Gene Name | Sequence Accession Number | Primer Sequence | Primer Location (Exon | Prod. Length | T°a | |
|---|---|---|---|---|---|---|
| WNT7A | Wingless-type MMTV integration site family, member 7A | NM_004625 |
F CAAAGAGAAGCAAGGCCAGTA R GTAGCCCAGCTCCCGAAACTG |
707-730 (3) 962-983 (4) | 277 | 60 |
| MYC | V-myc myelocytomatosis viral oncogene homolog (avian) | NM_002467.4 |
F CCAGCGCCTTCTCTCCGTC R GGGAGGCGCTGCGTAGTTGT |
1208-1226 (2) 1490-1509 (3) | 302 | 60 |
| JUN | Jun proto-oncogene | NM_002228.3 |
F TGGAAAGTACTCCCCTAACCT R CTGAAACATCGCACTATCCTT |
2786-2806 (1) 3015-3035 (1) | 250 | 60 |
| FRA-1 | FOS-like antigen 1 | NM_005438.3 |
F AGGAACCGGAGGAAGGAACTG R TGCCACTGGTACTGCCTGTGT |
554-574 (3) 732-752 (4) | 199 | 60 |
| AXIN2 | Axin 2 | NM_004655.3 |
F AAAAAGGGAAATTATAGGTATTAC R CGATTCTTCCTTAGACTTTG |
2678-2701 (10/11) 2935-2954 (11) | 277 | 54 |
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | NM_002046.3 |
F CACTGCCACCCAGAAGACTGTG R TGTAGGCCATGAGGTCCACCAC |
645-666 (8) 1072-1089 (9) | 449 | 63 |
| RPL32 | Ribosomal protein L32 | NM_000994.3 |
F GACTTGACAACAGGGTTCGTAG R ATTTAAACAGAAAACGTGCACA |
213-234 (3) 511-532 (4) | 320 | 60 |
| RPS18 | Ribosomal protein S18 | NM_022551.2 |
F CGATGGGCGGCGGAAAA R CAGTCGCTCCAGGTCTTCACGG |
105-121 (2) 366-387 (5) | 283 | 58 |
| B2M | Beta-2-microglobulin | NM_004048.2 |
F GAGGCTATCCAGCGTACTCCAA R CACACGGCAGGCATACTCAT |
115-136 (1/2) 347-366 (2) | 252 | 58 |
| ACTB | Actin beta | NM_001101.3 |
F TCCGCAAAGACCTGTACG R AAGAAAGGGTGTAACGCAACTA |
950-967 (5) 1226-1247 (6) | 298 | 60 |