Figure 4

Functional roles of SNCG in LNCaP-AI cells are ligand-dependent. (A) LNCaP-AI cells were transfected with full-length SNCG cDNA plasmid or empty vector as the control and selected with puromycin treatment. A stable SNCG-overexpressing clone (RFP-SNCG) and a negative control clone (RFP) were used for the subsequent experiments. RT-PCR and western blot analysis showed the SNCG expression in RFP-SNCG, LNCaP-AI and RFP cells. (B) PSA mRNA expression in RFP-SNCG with androgen administration by RT-PCR compared to RFP. (C) RT-PCR was used to detect the AR mRNA expression in RFP-SNCG with or without DHT administration compared to RFP. (D) Dual-luciferase reporter assays were performed to show the AR transcriptional activity in LNCaP-AI cells with treatment of androgen. (E) The cellular proliferation was detected by CCK-8 assay in RFP-SNCG with or without DHT treatment compared to RFP. ^* P < 0.05, ^** P < 0.01.