Figure 1

Serum starvation sensitizes ZL55cancer cells to CDDP. Results of flow cytometry analysis of ZL55 cells untreated (A) or serum-starved for 24 hours (B) are shown. C, the quantification of flow cytometry results of ZL55 cells of the untreated control, or treated with CDDP alone, serum starvation alone, or both together is shown (n=3). D, colony formation assay was performed after control treatment (Ctrl), treatment with CDDP or serum starvation (S) alone, and after the combined treatment (S/CDDP) (n=6, ** p<0.001). The quantification of the anti-pATM-S1981 immuno-staining of ZL55 cells (** for P<0.001) is shown in (E) (n=3). Results of Western blot with antibodies against pATM-S1981 (pATM), ATM, phospho Chk2-T68 (pChk2-T68), Chk2 and p53-Ser15 (p53-S15) and p53 for protein extracts are shown in (F).β-Actin and vinculin were used as loading controls.