Table 1 Conditions of PCR reactions: oligonucleotide sequences, annealing temperatures, number of cycles of the reactions, and amplicon size
From: Alterations in epidermal growth factor receptors 1 and 2 in esophageal squamous cell carcinomas
Genes | Oligonucleotide sequences (5²-3²) | ATa | Cycles | Amplicon size (bp) | Reference | |
|---|---|---|---|---|---|---|
EGFR – exon 18 | S | F: CAAATGAGCTGGCAAGTGCCGTGTC | 56°C | 35 | 400 | [22] |
AS | R: GAGTTTCCCAAACACTCAGTGAAAC | |||||
EGFR – exon 19 | S | F: GCAATATCAGCCTTAGGTGCGGCTC | 58°C | 35 | 372 | [22] |
AS | R: CATAGAAAGTGAACATTTAGGATGTG | |||||
EGFR – exon 20 | S | F: CCATGAGTACGTATTTTGAAACTC | 54°C | 35 | 408 | [22] |
AS | R: CATATCCCCATGGCAAACTCTTGC | |||||
EGFR – exon 21 | S | F: TAACGTTCGCCAGCCATAAGTCC | 58°C | 35 | 414 | [22] |
AS | R: GCTGCGAGCTCACCCAGAATGTCTGG | |||||
β-actin | S | F: GATGAGATTGGCATGGCTTT | 55°C | 40 | 100 | b |
AS | R: CACCTTCACCCGTTCCAGTTT | |||||
BRAF | S | F: CCTTTACTTACTACACCTCAGATA | 54°C | 40 | 189 | b |
AS | R: AATCAGTGGAAAAATAGCCT | |||||
KRAS | S | F: TGATAGTGTATTAACCTTATGTGTGAC | 54°C | 40 | 170 | b |
AS | R: TCTATTGTTGGATCATATTCGTC | |||||
GAPDH | S | F: CAACAGCCTCAAGATCATCAGCAA | 60°C | 40 | 123 | b |
AS | R: AGTGATGGCATGGACTGTGGTCAT | |||||
EGFR | S | F: TAACAAGCTCACGCAGTTGG | 60°C | 40 | 178 | b |
AS | R: GTTGAGGGCAATGAGGACAT | |||||
HER2 | S | F: CTCCTGTGTGGACCTGGATGAC | 60°C | 40 | 143 | b |
AS | R: GCTGCCGTCGCTTGATGA |