Curcumin and RL197 disrupt miR-ZBTB10/ZBTB4 interactions. RKO cells were treated with DMSO, curcumin, and RL197 alone or in combination with GSH, and induction of ZBTB10 or ZBTB4 (A) and downregulation of multiple miRs (B) were determined by real time PCR as outlined in the Materials and Methods. Results are means ± S.E. for at least 3 replicate determinations and significant (p < 0.05) induction of ZBTB10 or ZBTB4 and suppression of miRs (*) and inhibition of these responses by GSH (**) are indicated. (C) Curcumin and RL197-dependent downregulation of Sp1, Sp3 and Sp4 mRNA levels was determined in RKO cells as outlined in the Materials and Methods. Results are means ± S.E. (3 replicates) and significant (p < 0.05) inhibition is indicated. (D) Proposed mechanism of action of curcumin and RL197 in RKO cells.