Cooperation between GNF-2 and AKIs in inhibiting proliferation and clonigenicity of Ba/F3 JAK2 V617F cells. (A). Effect of GNF-2 and sub-optimal concentrations of AKIs on the observed IC50s using Ba/F3 JAK2 V617F cells. (B). Percentage of viable cells (+/− SD) treated for 72 h with AKIs at the indicated concentrations. Effect of GNF-2 with Imatinib (C), Nilotinib (D) and Dasatinib (E) on the anchorage-independent growth of Ba/F3 JAK2 V617F cells was evaluated using a colony forming assay, and the concentrations inhibiting clonigenicity by 50% (IC50) were calculated. P-values * p<0.05, ** p<0.005.