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Figure 6 | BMC Cancer

Figure 6

From: Fibroblast growth factor 19 expression correlates with tumor progression and poorer prognosis of hepatocellular carcinoma

Figure 6

Suppression of FGF19 expression was confirmed at transcript level by RT-PCR. (A) and at protein level by ELISA analysis (B). Analysis was performed at 48 h and 72 h following siRNA transfection for mRNA and protein level, respectively. FGF19 mRNA expression was suppressed to 30% of control siRNA in FGF19-specific siRNA-treated cells. n = 12, P < 0.05 at top of the Figure 6A, 6B, and 6C (like figure 6E and 6D). FGFR4 gene silencing by siRNA. Suppression of FGFR4 expression was confirmed at transcript level by RT-PCR (C) and at protein level by flow cytometry (D). Analysis was performed at 48 h and 72 h following siRNA transfection for mRNA and protein levels, respectively. FGFR4 mRNA expression was suppressed to 40% of control siRNA in FGFR4-specific siRNA-treated cells. n = 12, P < 0.05 at top of the Figure 6A, 6B, and 6C (like figure 6E and 6D). (E) Proliferation assay in JHH7 cells at 72 h after FGF19/FGFR4 siRNA treatment. (F) Apoptosis assay in JHH7 cells at 96 h after FGF19/FGFR4 siRNA treatment. PI, Proliferation Index. AI, Apoptosis Index.

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