Salinomycin induces EMT but decreases invasive properties. (A-C) The RT-qPCR data shows a decrease gene expression in E-cadherin and an upregulation of Snail and Zeb-1 as labeled, which correspond to an induction of EMT. All data is relative to the control of 0 μM salinomycin and endogenous control GAPDH. (D) Western blotting confirms the induction of Snail and shows an upregulation of the mesenchymal marker vimentin. (E) Micrographs of JLO-1 upon treatment with 2 μM salinomycin depicts alterations in cell morphology. (F) The graph denotes the fold change in number of cells that invaded through a matrigel membrane relative to the control of 0 μM salinomycin. Error bars represent standard deviation.