Isolation of HNSCC stem cell culture. (A) Flow cytometry confirms that our isolated cell culture is 91.5% CD44 positive. A nonspecific IgG antibody was used as a negative control. (B) RT-qPCR further confirms the stem cell characteristics of JLO-1 by showing elevated ALDH levels in comparison to three control cell lines (UMSCC-10B, HN1, and HN30). JLO-1 also has increased levels of Oct-4 and Nanog relative to UMSCC-10B.