|
TaqMan Buffer System
|
|---|
|
Primers
|
Parameter
|
EpiTect MethyLight Mix (Qiagen)
|
iQ Multiplex Powermix (Bio-Rad)**
|
Genotyping Master Mix (ABI)
|
QuantiTect Multiplex Mix (Qiagen)
|
|---|
|
ACTB
|
Cq
|
27.19±0.093
|
26.712±0.072
|
28.448±0.933
|
27.972±0.032
|
| |
ΔRn
|
0.244±0.004
|
2.655±0.003
|
0.791±0.090
|
0.170±0.003
|
|
CADM1
|
Cq
|
28.216±0.130
|
27.485±0.038
|
28.463±0.341
|
28.888±0.103
|
| |
ΔRn
|
0.267±0.006
|
2.722±0.038
|
1.160±0.056
|
0.182±0.004
|
|
MAL
|
Cq
|
26.432±0.170
|
25.593±0.030
|
29.499±0.837
|
26.759±0.053
|
| |
ΔRn
|
0.371±0.022
|
2.662±0.026
|
1.003±0.203
|
0.282±0.023
|
|
Hsa-miR-124-2
|
Cq
|
27.062±0.164
|
25.861±0.144
|
29.330±0.826
|
27.545±0.021
|
| |
ΔRn
|
0.630±0.010
|
6.505±0.029
|
0.691±0.125
|
0.514±0.014
|
-
* Triplicate analyses of 50 ng of bisulfite treated SiHa DNA. Mean values are indicated ± standard deviations.
-
** Threshold 0.1.
