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Table 1 Multiplex qMSP results using different TaqMan buffer systems *

From: Development of a multiplex methylation-specific PCR as candidate triage test for women with an HPV-positive cervical scrape

TaqMan Buffer System
Primers Parameter EpiTect MethyLight Mix (Qiagen) iQ Multiplex Powermix (Bio-Rad)** Genotyping Master Mix (ABI) QuantiTect Multiplex Mix (Qiagen)
ACTB Cq 27.19±0.093 26.712±0.072 28.448±0.933 27.972±0.032
  ΔRn 0.244±0.004 2.655±0.003 0.791±0.090 0.170±0.003
CADM1 Cq 28.216±0.130 27.485±0.038 28.463±0.341 28.888±0.103
  ΔRn 0.267±0.006 2.722±0.038 1.160±0.056 0.182±0.004
MAL Cq 26.432±0.170 25.593±0.030 29.499±0.837 26.759±0.053
  ΔRn 0.371±0.022 2.662±0.026 1.003±0.203 0.282±0.023
Hsa-miR-124-2 Cq 27.062±0.164 25.861±0.144 29.330±0.826 27.545±0.021
  ΔRn 0.630±0.010 6.505±0.029 0.691±0.125 0.514±0.014
  1. * Triplicate analyses of 50 ng of bisulfite treated SiHa DNA. Mean values are indicated ± standard deviations.
  2. ** Threshold 0.1.
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