Figure 2

Standard curves of CADM1 and MAL in a multiplex qMSP with ACTB ^* tested on a serial dilution series of methylation positive SiHa DNA in methylation negative DNA of primary keratinocytes. Standard curve of CADM1 (A) and MAL (B) with the previously described primers ⁹. CADM1 (C) and MAL (D) primers with nearly identical annealing temperatures as ACTB primers. For the dilution series the PCR efficiencies were calculated by E=(10^(−1/Slope)-1)×100%. The qPCR efficiencies increased from 76% to 87% for CADM1 and 52% to 105% for MAL.^*) Multiplex qMSP was performed with an ACTB primer concentration of 200 nM.