Figure 3

Effects of Buserelin on RhoA GTP expression and cell adhesion to Collagen I in MDA cells. A. Representative autoradiogram from Western blots showing the effects of Buserelin on RhoA expression in MDA cells transfected with the empty vector, the WT GnRHR and the GnRH-DesK191 cDNAs (lanes 1 to 3) or co-transfected with the WT GnRHR and p190RhoGAP or GAP-Y1284D cDNAs (lanes 4 and 5, respectively). B. Densitometric analysis of Rho GTP activity as determined by pull-down assays and Western blotting of extracts from cells transfected or co-transfected with the different expression plasmids and exposed to 10^-7M Buserelin or vehicle. C. Assessment of adhesion to Collagen I in cells transfected or co-transfected with the different GnRHRs and GAP expression plasmids described in A and exposed to Buserelin or vehicle. D. Relative F-actin levels in the cell adhesion experiments shown in C, as disclosed by fluorometry. The results shown in B, C, and D are the means ± SEM from 3 independent experiments. *p<0.05; **P < 0.01; ***p< 0.001.