Doxorubicin-enriched, ALDHbr mouse breast cancer stem cells are treatable to oncolytic herpes simplex virus type 1

Table 1 The primers used for the construction of pdICP34.5 and pdICP47 are listed

Primer name	Sequence
ICP34.5USf	¹⁵¹⁴⁵⁸CTCTGACCTGAGATTGGCGGCACTG¹⁵¹⁴⁸²
ICP34.5USr	GCGGCCGCAGCGCTGCGGCCGC ⁶⁴⁴CGCGGGCGCGCTCCTGACCGCGGG⁶²¹
ICP34.5DSf	GCGGCCGCAGCGCTGCGGCCGC ¹⁴²⁶CAGCGCGGCGGGGCCCGGCCAACCA¹⁴⁵⁰
ICP34.5DSr	²⁸⁹⁵TTCTTCCCTCTTCTCCCGCCCTCCA²⁸⁷¹
ICP47USf	AAAAGAATTCGAT¹⁴³⁶⁷⁵TGGGTTCGATTGGCAATGTTGTCTC¹⁴³⁶⁹⁹
ICP47USr	AAAAACTAGTGAT¹⁴⁵³¹⁰GTCCCGGGTACGACCATCACCCGAG¹⁴⁵²⁸⁶
ICP47DSf	AAAAAAGCTT ¹⁴⁵⁵⁷⁰CACGACATGCTCCCCCCCGACGAGC¹⁴⁵⁵⁹⁴
ICP47DSr	AAAACAGCTG ¹⁴⁶⁹⁸⁰ACGCGGAACTAGCGCGGACCGGTCG¹⁴⁶⁹⁵⁶
Linker 1	*CTAGT*GAATTCTAGTGGATCCCCCGGGCTGCAGGAATTCGATATCA
Linker 2	*AGCTT*GATATCGAATTCCTGCAGCCCGGGGGATCCACTAGAATTCA

The genome coordinates for the underlined genomic sequences are indicated. The restriction sites used for the construction of the shuttle plasmids are in bold italics. The reverse-complement sequences present in ICP34.5USr and ICP34.5DSf for overlapping PCR to join ICP34.5 US and DS FLRs are in bold.

ISSN: 1471-2407