Figure 3

Western blot analysis demonstrated increased P-gp expression in 4T1 ALDH ^br cells. The western blot analysis was performed as described in the Methods section. P-gp (141 kDa) expression was significantly increased in 4T1 ALDH^br cells compared with ALDH^lo cells. β-actin served as the loading control. The experiment was performed in duplicate with similar findings obtained in each experiment. A representative blot is shown.