Figure 2

Examples from screening of mCRC samples using the c.34 G > T CADMA assay. A. Real-time amplification data. High background fluorescence can be observed for sample ID 69. B. The derivative of the raw melting data (melt curve analysis). Sample ID 56 carry the c.34 G > T mutation. Sample ID 2, 69 and 72 were negative for the c.34 G > T mutation. Sample ID 69 and 72 may carry another KRAS mutation as small deviations from the wild-type replicates can be observed. Sample ID 69, which gave high fluorescence during the PCR amplification, has deviating melt curves from 82 to 88°C. C. Normalized HRM difference graph. Of the mCRC samples shown only one (sample ID 56) deviates more from the wild-type replicates than the standard containing 1% mutant alleles.