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Figure 2 | BMC Cancer

Figure 2

From: Development of a gene therapy strategy to target hepatocellular carcinoma based inhibition of protein phosphatase 2A using the α-fetoprotein promoter enhancer and pgk promoter: an in vitro and in vivo study

Figure 2

Specific overexpression of DN-PP2Acα using the AFP enhancer/ pgk promoter ( AFpg promoter) in AFP-positive HCC cells. (A) The transcriptional activities of the SV40, pgk and AFpg promoters in L-02, SK-Hep-1, HepG2 and Hep3B cells were tested using the luciferase reporter gene assay. (B) Construction of the DN-PP2Acα expression vector driven by the AFpg promoter. (C) Construction of the shuttle plasmids for preparation of recombinant adenoviruses. (D) Adenovirus-mediated gene transfer efficiency. Cells were infected with Ad-AFpg-luciferase at various MOI levels. At 24 h post-infection, a luciferase activity assay was performed. (E) Western blot analysis of DN-PP2Acα expression after infection of cells with recombinant adenoviruses at a MOI of 100.

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BMC Cancer

ISSN: 1471-2407

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