Figure 4

MiR-99a suppresses tumor growth in vivo. (A) 786-O cells were subcutaneously injected into nude mice to form solid, palapable tumors (day 25), following which synthetic miR-99a or NC mimics were intratumorally delivered for 4 weeks. Tumor volumes following miR-99a administration were significantly reduced compared with the control mice. (B) After tumor xenografts were intratumorally delivered synthetic miR-99a or NC mimics for 4 weeks, we extraced the protein and performed Western blot. We found that intratumoral delivery of synthetic miR-99a induced a makedly inhibition of mTOR expression compared with control mice. Data are represented as mean ± SD. *, P < 0.05. **, P < 0.01.