Figure 1

MiR-99a is downregulated in renal cell carcinoma. (A) Real-time qRT-PCR analysis of relative miR-99a expression levels in RCC cell lines (786-O and OS-RC-2) and normal immortalized renal cell line (HK-2). (B) Relative miR-99a expression levels in 40 pairs of RCC and their matched adjacent non-tumor tissues as assessed by real-time qRT-PCR. MiR-99a was considered to be significantly downregulated only if the calculated fold-change was less than 0.5 in the tumor tissue compared with the matched adjacent non-tumor tissue. (C) Correlation of miR-99a expression with overall survival in RCC patients. Overall survival of RCC patients were analyzed by Kaplan-Meier analysis in SPSS 17.0. Relative miR-99a level was assessed by real-time qRT-PCR and T/N = 0.5 was chosen as the cut-off point for separating miR-99a high-expression tumors (n = 11; T/N > 0.5) from miR-99a low-expression cases (n = 29; T/N < 0.5). Data were normalized to U6 control and are represented as mean ± standard deviation (SD) from three independent experiments. T means RCC tissues. N means matched adjacent non-tumor tissues. **, P < 0.01.