Figure 4
From: PTPRZ1 regulates calmodulin phosphorylation and tumor progression in small-cell lung carcinoma
PTPRZ1 regulates tyrosine phosphorylation in SCLC cells. A, Tyrosine phosphorylated proteins in H69 cells were detected by Western blot with anti-pTyr antibody (4 G10) with β-catenin as loading control on top. B, Tyrosine phosphorylated CaM was detected by Western blot with anti-phosphorylated CaM, with total CaM on top as loading control in H69 cells. C and D, shZ1s block PTN-stimulated tyrosine phosphorylation of CaM in H69 (C) and H1930 (D). PTPRZ1 promotes the phosphorylation of calmodulin in response to PTN. E, Correlated expression of PTPRZ1 and tyrosine phosphphorylated CaM (pCaM) in human lung NET tissues. Representative negative (−) or positive (+) images were shown. Scale bars were 20 μm (Magnification X40). P < 0.05 was considered statistically significant.