Figure 3

PTPRZ1 expression was downregulated by shRNA in SCLC cell lines. A, shRNA targeting PTPRZ1 (shZ1) successfully knock-downed PTPRZ1 mRNA by up to 75% in the SCLC cell lines, H69 and H1930, as compared to negative control construct expressing shLUC. White bars = shLUC, dotted bars = shZ1#1, gray bars = shZ1#2, black bars = shZ1#3. Error bars represent SD. Asterisk denotes P < 0.05 using Student’s t test, while NS denotes non-significant change. B, PTPRZ1 downregulation in H69 and H1930 was confirmed by Western blot, using β-tubulin as control for protein levels. C, FACS analysis of surface PTPRZ1 protein expression on H69 and H1930 cells, with shZ1 down-regulating PTPRZ1 expression levels.