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Figure 10 | BMC Cancer

Figure 10

From: Tumorigenic potential of pituitary tumor transforming gene (PTTG) in vivoinvestigated using a transgenic mouse model, and effects of cross breeding with p53 (+/−) transgenic mice

Figure 10

Representative genotyping of offspring from TgPTTG crossbred to p53 +/− mice. Lane 1, ladder; lane 2, empty; lane 3–9, individual mice samples (ID# 487–495); lane 10 is negative control of PCR reaction mix without template DNA; and lane 11, positive control. p53+/− mice were genotyped using recommended primers from Jackson labs resulting in a robust 400 kb band for p53+ and a weaker 600 kb band for p53-. Mice were then cross-referenced to PTTG-EGFP genotype.

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