Figure 1

Role of Snail in invasion and migration of gastric cancer cell lines. A. SNU216 (upper panel) and SNU484 (lower panel) cells were infected with lentiviruses expressing either non-target shRNA (shNT) or Snail shRNA on day 0, and then harvested on day 7 post-infection. Snail knockdown was determined by RT-PCR and western blotting; stable cell lines were generated for each of the cell lines (sh-Snail). Silencing of Snail in SNU216 and SNU484 cells induced decreased migration and invasion. B. SNU216 (upper panel) and SNU484 (lower panel) cells were infected with lentiviruses expressing either a lentiviral PLKO vector targeting Snail or an empty PLKO vector (EV) on day 0, and then harvested on day 7 post-infection. The overexpression of Snail was determined by RT-PCR and western blotting; stable cell line was generated for each of the cell lines (O/E-snail). Snail overexpression in SNU216 and SNU484 cells induced increased migration and invasion. C. Snail overexpression induced increased mRNA expression of VEGF and MMP11 in SNU216 and SNU484 cells in real-time RT-PCR analysis. Lower panel indicates representative RT-PCR figures for VEGF, MMP11, Snail, and GAPDH. Data show the mean ± SE of at least 3 independent experiments. * indicates P < 0.05 by Student’s t-test.