Figure 6

DNA vector-mediated PLK1 overexpression could partially rescue the effects of miR-100 mimics on malignant phenotypes of A549 cells. (A) RT-PCR and Western Blot analysis of PTEN mRNA and protein expression in A549/miR-NC, A549/miR-100 or A549/miR-100 co-transfected with pcDNA/control or pcDNA/PLK1. (B) MTT analysis of growth in A549/mi-NC, A549/miR-100 or A549/miR-100 co-transfected with pcDNA/control or pcDNA/PLK1. (C) Hoechst staining analysis of apoptosis in A549/miR-NC, A549/miR-100 or A549/miR-100 co-transfected with pcDNA/control or pcDNA/PLK1. The percentage of Hoechst-positive nuclei per optical field (at least 50 fields) was counted. (D) Flow cytometric analysis of cell cycle distribution in A549/miR-NC, A549/miR-100 or A549/miR-100 co-transfected with pcDNA/control or pcDNA/PLK1. * indicates P < 0.05. The data are expressed as the mean value ± SEM of the results obtained from three independent experiments. Corresponding P values analyzed by ANNOVA tests are indicated.