Figure 3

Effects of miR-100 mimics or inhibitor on growth, apoptosis and cell cycle of NSCLC cells. (A) 48h after A549 cells were transfected with miR-100 mimics (or miR-NC mimics) or anti-miR-100 (or anti-miR-NC), Taqman real-time quantitative RT-PCR analysis of miR-100 expression. (B) MTT analysis of growth in A549/miR-100 (or A549/miR-NC) or A549/anti-miR-100 (or A549/anti-miR-NC) cells. (C) Hoechst staining analysis of apoptosis in A549/miR-100 (or A549/miR-NC) or A549/anti-miR-100 (or A549/anti-miR-NC) cells. Fragmentation of the nucleus into oligonucleosomes and chromatin condensation were examined by fluorescence microscopy. The percentage of Hoechst-positive nuclei per optical field (at least 50 fields) was counted. (D) Flow cytometric analysis of cell cycle distribution in A549/miR-100 (or A549/miR-NC) or A549/anti-miR-100 (or A549/anti-miR-NC) cells. * and ** indicate P < 0.05 and P < 0.01, respectively. Each experiment was performed at least in triplicate. Corresponding P values analyzed by t-tests are indicated.