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Figure 4 | BMC Cancer

Figure 4

From: Junction region of EWS-FLI1 fusion protein has a dominant negative effect in Ewing’s Sarcoma in vitro

Figure 4

Bacterial expression and purification of Junction region (aa 251- aa 343) . A: Coomassie stained SDS PAGE gel picture of Ni-NTA Purification of Junction- EWS-FLI1 protein under native condition. Lane1: Protein ladder, Lane 2: Induced cell lysate soluble fraction, Lane 3: Flow Through, Lane 4: Wash and Lane 5: Elute. Figure 4 B: Confirmation of junction region (aa 251–343) protein expression by immunoblotting with anti Thio antibody. Lane 1: insoluble fraction, Lane 2: soluble fraction. Figure 4 C: CD of junction region (aa 251-aa 343) of EWS-FLI1 soluble construct.

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