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Figure 2 | BMC Cancer

Figure 2

From: Loss of miR-133a expression associated with poor survival of breast cancer and restoration of miR-133a expression inhibited breast cancer cell growth and invasion

Figure 2

Regulation of breast cancer cell growth, invasion and migration by miR-133a. A. MCF-7 cells were grown and transiently transfected with miR-133a mimic or negative control, and cell proliferation was then assessed afterwards. The experiments were performed in triplicate and repeated thrice. B. MCF-7 cells were grown and transiently transfected with miR-133a ASO or negative control, and cell proliferation was assessed afterwards. The experiments were performed in triplicate and repeated thrice. C. Transwell migration and Matrigel invasion assays. MDA-MB-231 and MCF-7 cells were grown and transiently transfected with miR-133a mimic or miR-133a ASO for 2 days and subjected to migration and invasion assays. Representative photographs (upper) and quantification (lower) are shown. Magnification: × 200. D. Wound healing assay. Cells were transfected with ASO NC or miR-133a ASO for 72 h. Images were taken at 0, 24, and 48 h. Quantification of cell motility by measuring the distance between the invading front of cells in three random selected microscopic fields for each condition and time point. The degree of motility is expressed as percent of wound closure as compared with the zero time point. Magnification: × 100. * P < 0.05; ** P < 0.01.

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