Figure 2

PCA3 knockdown inhibits LNCaP cell growth and survival. (A) LNCaP cells were transfected with 60 nM of siPCA3 and non-targeting siRNA (siScr), and then followed for cell proliferation for 96 h. (B) NIH3T3 and (C) HeLa cells were transfected with 60 nM of siPCA3 or non-targeting siRNA (siScr) and then followed for cell growth for 48 h. Cell growth was evaluated using the crystal-violet assay described in the Methods section. Data represent the mean ± SD of three independent experiments (* p < 0.05). (D) The effect of PCA3 knockdown on LNCaP cell viability was measured using Trypan Blue exclusion analysis. Data are shown as the mean ± SD of three experiments.