Figure 5
From: Identification of PADI2 as a potential breast cancer biomarker and therapeutic target
HER2/ERBB2 expressing cell lines BT-474 and SK-BR-3 show decreased proliferation after treatment with Cl-amidine. (a) BT-474 and SK-BR-3 cell lines were treated with increasing concentrations of Cl-amidine (0 μM, 100 μM, 200 μM, and 400 μM) over 4d and analyzed by flow-cytometry. Cell counts show a dose-dependent decrease in the growth of both BT-474 and SK-BR-3 (* p < 0.05, ** p < 0.001). (b) Apoptosis levels (cleaved Caspase-3 positive) significantly increase over the control in both BT-474 and SK-BR-3 cells after 200 μM and 400 μM of Cl-amidine. Tunicamycin (10μg/mL ) is shown as a control for apoptosis (* p < 0.05, ** p < 0.01, *** p < 0.001). (c) Cell cycle analysis of Cl-amidine treated cells (DAPI) indicates S-phase arrest occurs in both BT-474 and SK-BR-3 cell lines. Data are expressed as the mean ± SD from three independent experiments.