A closed-tube methylation-sensitive high resolution melting assay (MS-HRMA) for the semi-quantitative determination of CST6 promoter methylation in clinical samples

Table 2 Contingency table which tabulates the outcomes of both methods for all samples tested and kappa index values (n = 116)

Method		MSP		Total
		pos	neg
MS-HRM	pos	34	10	44
	neg	22	50	72
total		56	60	116
Indices of agreement for MS-HRMA and MSP for *CST6* methylation
Agreement index	Type of agreement	Calculated values	Standard error	CI (95%)
p_o	overall	0.7241
p_pos	positive	0.6800
p_neg	negative	0.7575
p_e	chance	0.5041
Kappa index	Chance corrected	0.4436	0.0817	0.2834 – 0.6038

ISSN: 1471-2407