Figure 6

A 132 bp DNA region is responsible for repression of PTEN promoter by Tbx3. 132 bp from the 5^′-end of CP-PTEN was deleted to generate the Δ mut. CP-PTEN. All three reporter constructs were transfected into HeLa cell line and promoter activities were determined in the absence or presence of 1μg Tbx3 expressing plasmid. As has been shown before, Tbx3 was capable of repressing promoter activities of both the EP-PTEN and CP-PTEN, whereas no repression by Tbx3 was detected in Δ mut. CP-PTEN. Western blot (WB) shown below was performed by an anti-Tbx3 antibody and displays the Tbx3 protein in untransfected and transfected cells.