Impact of TTP in the TUM-CAM model on tumour proliferation and apoptosis A: Using the proliferation marker Ki-67 there were not significant differences in proliferation of total tumour specimens (Figure
A, p= 0.9355). B: Analyzing the upper 100 μm of tumour tissue argon gas had no significant effect on proliferation. 10s TTP highly significantly decreased proliferation (p< 0.0001), with 20s TTP showing no additional effects (10s versus 20s p= 0.9359). C: TTP induced highly significant apoptosis after 10 seconds (p< 0.0001), and after 20 seconds (p= 0.0006) in the upper 100 μm of the tumour. D: Depth of effective tissue penetration (DETiP) of 20s TTP was up to about 60μm (p<0.0001), with 10s TTP not being significantly different (p=0.1428).