Representative IHC staining of ECM1. A-D: ECM1 was detected primarily in the cytoplasm of breast cancer cells (200×): A. ECM1 negative; B. + ECM1 staining; C. ++ ECM1 staining; D. +++ ECM1 staining. E-F normal breast epithelium (200×): E. ECM1 negative; F. breast ductal epithelial cells with cytoplasmic ECM1 staining classified as +. G-H lymph node metastases: G. metastatic cells with ECM1 staining (200×) classified as +; H. cytoplasm of metastatic cells with ECM1 staining (100×) classified as ++.