Figure 6
Salinomycin causes accumulation of human CC cells in the G2-phase. Mz-ChA-1, TFK-1 and EGI-1 cells were plated in 12-well plates with 1 x 105 cells/well in culture medium. Cells were grown until confluence and then further cultured in medium (A) or presence of 1 mM Gemcitabine (B), 5 μM (C) and 10 μM Salinomycin for 24 hours (D). Cells were trypsinized and cell cycle was analyzed by flow cytometry. In Mz-ChA-1 and TFK-1 cells, increasing concentrations of Salinomycin resulted in accumulation of cells in the G2-phase. In contrast, differences in cell cycle phases were hardly observed in EGI-1 cells treated with Salinomycin. Results are shown as summary of 3 independent experiments as mean ± SD.