Figure 4
Impaired migration after treatment with Salinomycin. Tumor cell migration was analyzed using transwell chambers provided with an 8 μm pore polycarbonate membrane. Mz-ChA-1, TFK-1 and EGI-1 cells were placed at 5 x 105 cells/well in culture medium containing 10% fetal calf serum in the upper compartment of the chamber. As chemoattractant, the lower compartment was filled with culture medium containing 30% fetal calf serum. Cells were cultured in the absence or presence of 1 mM Gemcitabine, 1 μM, 2 μM, 5 μM or 10 μM Salinomycin for 48 hours. The membrane was then removed, fixed with ethanol and stained with hematoxylin. The membranes were analyzed under a light microscope counting the number of migrated cells to the lower surface of the membrane in five random fields. Treatment with Salinomycin resulted in impaired migration in all three tumor cell lines. Results are shown as representative pictures of light microscopic display (A) and as mean ± SD (B) of 3 independent experiments. * p < 0,05 compared with control.