Figure 6

Hyaluronan promotes growth, migration and invasion in TamR cells. Treatment of TamR cells which naturally overexpress CD44 with the CD44 ligand, HA, resulted in a small but non-significant gain in growth (A) and an accompanying activation of ERK1/2 (B) which was not seen in MCF7 cells (C, D). Treatment of TamR cells with HA also enhanced their migratory capacity, an effect not seen in MCF7 cells (E; p=0.05 versus untreated cells). siRNA-mediated inhibition of CD44 expression in TamR cells prevented HA-induced cell migration (F, p=0.05 versus CD44siRNA-treated cells); accompanying images are immunohistochemical staining of CD44 in NT- and CD44siRNA-treated TamR wound assays.