Table 1 LoB, LoD, linear dynamic range, PCR efficiency of the 12 PCR assays included in EndoPredict
Gene | LoB | LoD [Cq value] | Linear range [log2 dilution step] | Linear range [Cq value] | Efficiency [%] |
|---|---|---|---|---|---|
AZGP1 | 40.0 | 35.6 (34.2 - 36.4) | −13.1 to 0 | 35.6 to 20.5 | 81.9 (80.3 - 83.6) |
CALM2 | 40.0 | 35.4 (34.3 - 36.0) | −14.0 to 0 | 35.4 to 21.6 | 101.4 (99.8 - 103.2) |
BIRC5 | 40.0 | 36.3 (35.4 - 36.9) | −9.1 to 0 | 36.3 to 26.7 | 93.3 (90.5 - 96.2) |
DHCR7 | 40.0 | 36.3 (35.4 - 36.8) | −10.9 to 0 | 36.3 to 24.5 | 89.9 (87.6 - 92.3) |
IL6ST | 40.0 | 36.8 (35.7 - 37.5) | −11.5 to 0 | 36.8 to 23.3 | 80.7 (78.8 - 82.7) |
MGP | 40.0 | 37.2 (35.2 - 38.2) | −13.9 to 0 | 37.2 to 20.2 | 76.3 (74.4 - 78.2) |
OAZ1 | 40.0 | 36.6 (35.5 - 37.2) | −12.9 to 0 | 36.6 to 22.6 | 89.0 (87.6 - 90.4) |
RBBP8 | 40.0 | 35.6 (34.7 - 36.1) | −9.4 to 0 | 35.6 to 25.9 | 96.3 (93.2 - 99.6) |
STC2 | 40.0 | 35.1 (34.2 - 35.7) | −9.9 to 0 | 35.1 to 24.0 | 85.2 (82.6 - 87.9) |
UBE2C | 40.0 | 36.0 (34.9 - 36.7) | −10.1 to 0 | 36.0 to 24.4 | 83.3 (81.1 - 85.7) |
RPL37A | 40.0 | 36.0 (34.5 - 36.7) | −16.4 to 0 | 36.0 to 19.0 | 94.5 (92.9 - 96.1) |
HBBV2 | 40.0 | 35.3 (33.6 - 36.2) | −7.6 to 0 | 35.3 to 25.9 | 75.4 (70.1 - 81.6) |