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Table 1 Primer sequences and PCR settings

From: LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro

Gene name

Primer direction

Sequences (5’-3’)

Number of cycle

Hybridization temperature

CD11a

Forward

GGGAATGACCTTGGCAACAGACCCCACAGAT

34

58

 

Reverse

GGGTCTCCTGACTCTCCTTGGTCT

  

CD18

Forward

CATCAGAGCTGCTGTAGAGC

31

60

 

Reverse

GCTGACCTTGAACTTCGTGC

  

ICAM-1

Forward

TGCAGCACCTCCTGTGACCA

25

60

 

Reverse

CAGTTCCACCCGTTCTGGAG

  

E-Selectin

Forward

GGACTGCGTGGAGATCTACA

30

61

 

Reverse

AGCCAGGGTCACACTTGCAA

  

N-Cadherin

Forward

GAAGGATGTGCATGAAGGAC

30

56

 

Reverse

AGTTAAGGTTGGCTTCAGGC

  

GAPDH

Forward

GTCGTATTGGGCGCCTGGTCAC

15

60

 

Reverse

AGGGGCCATCCACAGTCTTCTG

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