Figure 1
Stimulation of purified NK cells with monocytes. Purified monocytes were co-cultured with purified NK cells (5 x 105 cells/well) in 1:1 cell-ratio (NK + Mo) for one and three days. (a) Expression of CD69 on NK cells, (b) IFN-γ-release and (c) cytotoxicity against ovarian cancer cell lines were determined by (a) flow-cytometry, (b) ELISA and (c) CD107a-degranulation-assay, respectively. Data are shown as means + standard error (SE) of three to six independent experiments. Statistical analysis was performed by unpaired t-test, statistical significance (p ≤ 0.05) is indicated.