Figure 8
From: Novel anti-HER2 monoclonal antibodies: synergy and antagonism with tumor necrosis factor-α
In vitro molecular responses of SK-BR-3 cell to anti-HER2 antibody BH1 and tumor necrosis factor-α. SK-BR-3 cells were treated up to 24 h with BH1 antibody (10 μg/ml), TNF-α (1000 U/ml) or the combination of both agents. Cell lysates were prepared from cells harvested at indicated times (hr, hours) and the expressions of phospho-HER2 (pHER2), phospho-Akt (pAkt), total Akt (Akt), phospho-ERK1/phospho-Erk2 (pErk1/2), total Erk1/Erk2 (Erk 1/2) and Cyclin D1 were analyzed by western blotting. Calnexin was used as a loading control. Untreated cells were incubated in a parallel experiment in the absence of any treatment.