Figure 7
From: Novel anti-HER2 monoclonal antibodies: synergy and antagonism with tumor necrosis factor-α
Anti-HER2 antibody and tumor necrosis factor-α interaction is cell type-specific. The growth of breast cancer cell lines under various combinations of BH1 antibody (0, 0.161, 0.312, 0.625, 1.25, 2.5, 5.0 μg/ml) and TNF-α (0, 8, 16, 32, 62.5, 125, 250, 500 U/ml) treatments was experimentally tested in triplicate and the average relative growth levels were calculated. Heatmap represents the cell growth (as measured by OD) under different conditions. Synergistic and antagonistic interactions are indicated by green and blue dots, respectively. Numbers associated with cell line names indicate interaction scores. According to this checkerboard assay, BH1 and TNF-α show synergy in SK-BR-3 cells, but antagonism in BT-474 and MDA-MB-361 cells. There is no interaction in MCF-7 cells.