Figure 4
From: Novel anti-HER2 monoclonal antibodies: synergy and antagonism with tumor necrosis factor-α
Epitope mapping of anti-HER2 antibodies using indirect immunofluorescence assay. Huh7 cells were transfected in 6-well plates using a set of mammalian expression plasmids encoding full-length or N-terminally truncated HER2 protein. Transfected cells were cultivated for 48 h, and subjected to indirect immunofluorescence assay. Transfected cells that were recognized by specific antibodies displayed strong green fluorescence. The letters from (a) to (k) indicate the plasmids in the following order: pDEST26 (empty vector), p219D, pCC2001, pCC2005, pCC2004, pCC2003, pCC2002, pCC2008, pCC2007, pCC2006, pCC2012 (see Figure 3 for description of HER2-expression plasmids). (−) No primary antibody, Tzm: Trastuzumab, CB11: a monoclonal antibody recognizing an epitope at intracellular domain of HER2 that was used as a positive control for expression of transfected plasmids.