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Figure 3 | BMC Cancer

Figure 3

From: Novel anti-HER2 monoclonal antibodies: synergy and antagonism with tumor necrosis factor-α

Figure 3

Schematic representation of antibody epitope mapping studies. Mammalian expression plasmids expressing full-length HER2 (p219D) or partially deleted HER2 were transfected into Huh7 cells. Anti-HER2 antibody reactivity was tested by indirect immunofluorescence (Figure 4) and immunoprecipitation-western blotting (Additional file 3) techniques. The boxes named as I, II, III, IV represent four subdomains of ECD. Grey box: transmembrane domain, TK: tyrosine kinase domain, CT: carboxyl-terminal domain. Tzm: Trastuzumab.+: antibody reactivity, -: no antibody reactivity. Stars (*) near plasmid names indicate that immunofluorescence data were confirmed with immunoprecipitation-western blot assay.

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