Figure 1

Effect of Slug knockdown on miR-221 expression in breast cancer cells. MDA-MB-231 (A, B) and MDA-MB-436 (C) breast cancer cells were transfected with 30nM si-Slug molecule or a non-relevant siRNA (si-Scr). Slug and miR-221 expression was determined at RNA level at three different times (24 h, 48 h, 72 h), and revealed by quantitative RT-PCR analysis. RT-PCR results were calculated using the ΔΔCt method and data are presented as fold change respect to control untreated cells (Ctr 24 h for MDA-MB-231, and Ctr 72 h for MDA-MB-436). Results represent means ± SEM of three independent experiments. p-values ≤ 0.05 were considered statistically significant. In panel A, Slug expression investigated at protein level and revealed by Western Blot, is reported. IP3K was used as loading control.