Figure 5

The rates of migration and invasion in CypA interfered 95C and A549 cells with A, Migration was investigated using a wound healing assay. The percent areas of wound closure at 24 h following wound generation were calculated from three separate areas and are expressed as the mean±SEM. **P<0.01 (t-test). Photomicrographs were taken after 24 h (original magnification, ×100). B, Other migration assays were performed using a Transwell unit. Cells on the underside of the insert filters were fixed, stained, and counted under a microscope. The data are expressed as means ± SEM of triplicate values from three separate experiments. **P<0.01 (t-test). Photomicrographs were taken after 24 h (original magnification, ×200). C, The in vitro invasive properties of 95C and A549 cells was tested using a Matrigel-coated Transwell unit. Cells on the underside of the insert filters were fixed, stained, and counted under a microscope. The data are expressed as means ± SEM of triplicate values from three separate experiments. **P<0.01 (t-test). Photomicrographs were taken after 48 h (original magnification, ×400).